Throughout history, from the genus Mentha L. and the family (Lamiaceae), species including approximately 260 genera and more than 7000 species are used throughout the world and an example is Mentha piperita L. (Brahmi, Khodir, Mohamed & Pierre, 2017) commonly known as peppermint a hybrid of spearmint (Mentha spicata L.) and water mint (Mentha aquatica L.) has a strong sweetish aroma and a warm, pungent flavor with a refreshing aftertaste (Encyclopedia Britannica, 2018). It is a perennial herb 50–90 cm tall, typically quadrilateral, the commonly branched stems are generally purplish but occasionally gray and the dark green or light green leaves are short, oblong-ovate and serrated with finely toothed margins while the flowers are purple or pinkish with false ears with numerous imperceptible bracts and rarely bear seeds or most of the time sterile and spreads via stolons and grows in sunny places and prefers acidic, neutral and basic, light, medium soils but can also grow in heavy clay soils ( Belkheir, Shushni, & Singh, 2015). It has been cultivated throughout Asia, Europe, and North America since it was introduced into the London Pharmacopoeia in 1721 thanks to its essential oil (Brahmi, Khodir, Mohamed, & Pierre, 2017) extracted from the aerial parts of the flowering plant, the dried leaves, the fresh flowering plant and the whole plant used as medicine (Belkheir, Shushni and Singh, 2015), while the dried flowers are used as a flavoring and the fresh leaves normally used as a culinary herb, (Encyclopaedia Britannica, 2018) as therapy for common cold, inflammation of the mouth, pharynx, liver, as well as disorders of the gastrointestinal tract such as nausea, vomiting, diarrhea, cramps, flatulence and dyspepsia. M. piperita L. has long been used as safe medicinal preparations, less toxic than synthetic ones, contains a wide variety of bioactive compounds that are an excellent source of phytochemicals, antioxidants, antinociceptive, anti-inflammatory, antimicrobial, anti-carcinogenic , antiviral, antiallergic and anticancer benefits in the prevention or treatment of numerous diseases. Furthermore, M. piperita L has great potential for cost-effective and cost-effective application in both the medical and food industries (Barbalho, Guiguer, Menezes, & Trevisan, 2017). Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essayMentha piperita L. produces 0.1–1% volatile oil composed primarily of menthol (29–48%), menthone (20–31%), mentholfuran (6.8%), and menthyl acetate (3– 10%). Other pharmacologically active ingredients include bitter substances, caffeic acid, flavonoids (12%), polymerized polyphenols (19%), carotenes, tocopherols, betaine, choline, and tannins (Singh, Shushni, & Belkheir, 2015). Levo-menthol, a left-handed form naturally obtained from menthol also known as peppermint camphor, a terpene alcohol with a strong odor and refreshing minty taste, is obtained from peppermint oil or is produced synthetically by hydrogenation of thymol. It is used medicinally in ointments, cough drops, nasal inhalers, and is also used as a flavoring in foods, cigarettes, liquors, cosmetics, and perfumes (Wade, 2018). Due to its pleasant minty flavor and refreshing sensation on the mouth, lungs and throat, menthol cigarettes "a cause of tooth decay (Dental Health & Your Health, 2016)" gained popularity in the 1950s and usually sold as "cigarettes" healthy" (Wickham, 2015), but is now shown by studies to be primarily responsible for an increasing number of youth (people aged 11-17) and young adults (people aged 18-30) smoking . Although there is little evidence to prove thatmenthol in cigarettes is the cause of nicotine addiction and unsuccessful smoking cessation, studies indicate that the refreshing and anesthetic properties of menthol sweeten and soften the taste of cigarettes pose a public health risk. As a sort of countermeasure to this alarming state of youth and young adult smokers, WHO created the Study Group on Tobacco Product Regulation (TobReg) which presents its first advisory note on menthol in tobacco products which it undoubtedly recommends to ban the use of menthol and its analogues, precursors or derivatives in cigarettes and possibly in all tobacco products (World Health Organization, 2016). This could be a great opportunity for members of the mint family to advance further and reclaim its primacy as a traditionally used herb and medicine to bridge the gap from the time it was abused and misused. Complete disappearance of teeth can sometimes take a toll on your health. smiling and cause degradation of self-esteem, self-esteem resulting in social withdrawal and difficulty eating one's favorite and adequate nutritional food therapy (Kalman, 2017). Tooth decay, one of the causes of imperfect smile is the loss of the outer layer of the tooth, the enamel and the calcified tissue surrounding the pulp cavity of the tooth, the dentin, which occurs when carbohydrate-containing food is neglected on the teeth and bacteria present in the mouth are digested and convert those foods into acids. Bacteria, acids, food debris, and saliva mix to form plaque that adheres to the tooth enamel surface, causing tooth decay (Friedman, 2017). Tooth decay is normal in children, but with age it becomes a problem for adults too. Breaking of the edges or margins of fillings is also common in older adults due to fluoride deficiency and modern prophylactic dental care during growth (American Dental Association, 2018). A survey shows that 45% out of 10,000 adults are unable to brush their teeth at night due to tiredness, forgetfulness and laziness, while 28% only use water to brush their teeth and 14% only use their fingers to wash yourself (Parry, 2015). So, we student pharmacists of the University of Negros Occidental - Recoletos want to formulate a mouthwash using the extracts of Mentha piperita L. leaf that perhaps could alleviate, save time and effort in people's lives and help minimize some causes of tooth decay in particular bacterial cause, as mouthwash helps to minimize and support low levels of bacteria, eliminate food residues and block bad breath in the mouth thanks to its fresh mint aroma (Parry, 2015). Charles Darwin, in his theory of natural selection, described “survival of the fittest” as the change of living organisms over time called evolution. Evolution, a biological phenomenon given to all living organisms that leads to the formation of new life forms supported by fossil records, structure, function, embryological development, DNA and RNA studies (Green, Joshi, & Rogers, 2018). To survive, organisms have formed toxic compounds as protection to fight and make progress for existence, and they have also created resistance for the toxins produced. Bacteria create infectious proteins for the host, such as diphtheria toxin, bacteriocins that are toxic to the bacteria. Toxins indirectly lead to modification through cell death which modifies cell division to stimulate transformation (Fridovich, Keil, & Robinson, 2018). So, in connecting this theory to our study, we will focus primarily on the growth inhibition of Streptococcus.