Use the same plate as the TSA strip. Determine whether catalase is produced through the breakdown of hydrogen peroxide. Proceed with the addition of the hydrogen peroxide reagent, paying attention to the bubbling. In other words, bubbling occurs due to the breakdown of hydrogen peroxide and the manufacture of oxygen gas (Benson, 2010). The control for this test is S. aureus. Blood agar plate A stripe is drawn on the plate for isolation. Also, check the plate for hemolysis. Look for the body's ability to produce hemolysin. Bacteria with hemolysin will generate different patterns. For starters, the green part around the colony is alpha. Secondly, it is best to clean the colony completely. Third, cloudiness or lack thereof is gamma. Controls are K. pneumoniae, B. cereus, and S. saprophyticus in order based on previous results. Simmons Citrate Agar Slant The use of this control is to find out whether or not an organism is capable of exerting citrate as a carbon source. Place a needle of your organism along the surface of the slope. So, start from the bottom and work your way up with the needle. The indicator already present in the media is bromothymol blue. An increase in PH will generate a bright blue for a positive result. The control is E.